Research Paper (Abstract)
Capillary gel electrophoresis (CGE) is a well-established separation technique for the analysis of biologically important molecules such as nucleic acids. The inherent high resolving power, rapid analysis times, excellent detection sensitivity and quantification capabilities makes this method favorable compared to conventional manual polyacrylamide (PAGE) and agarose slab gel electrophoresis techniques. In this paper we introduce a novel single-channel capillary gel electrophoresis system with light emitting diode induced fluorescence detection (LedIF) also utilizing a compact pen-shaped capillary cartridge design for automatic analysis of samples form a 96 well plate.
To evaluate the suitability of the system, 1000 genomic DNA samples were analyzed in gel filled capillaries and detected by the micro-ball ended excitation and emission optical fiber based LED induced fluorescence detection system. Excellent migration time reproducibility of RSD<0.75% was obtained over the course of 1000 runs. The system rapidly distinguished between intact and degraded genomic DNA samples, therefore provided important information if they could be used for downstream quantitative PCR (qPCR) processing where high-quality intact genomic DNA was key. We envision that this novel system design will rapidly find new applications in both research and clinical diagnostic labs as a highly sensitive and easy to use bio-analytical approach.
Source Link (ELECTROPHORESIS):
http://onlinelibrary.wiley.com/doi/10.1002/elps.201200139/abstract;jsessionid=FFA4C47AAD5D3F2436C237F4D8254D3A.d04t03
DOI: 10.1002/elps.201200139
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Looking forward to implement the same structure and knowledge in the same.
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